Figure 2

Total NO production and iNOS expression in adiponectin-stimulated osteoarthritis (OA) chondrocytes. Adiponectin significantly increased total NO production (n = 6, duplicate, P < 0.01) (A). Western blotting showed upregulation of iNOS expression in OA chondrocytes stimulated with 30 μg/ml adiponectin (B). Both nonselective (L-NMMA, 2 mM) and selective NOS inhibitors (L-NIL, 50 μM) significantly prevented total NO secretion (n = 4, duplicate, P < 0.05 for both) (c). The histogram with error bars represents the mean and the standard error of the mean. *P < 0.01 by Mann-Whitney test; †P < 0.05 by Mann-Whitney test.