Mosaic chromosomal aberrations in synovial fibroblasts of patients with rheumatoid arthritis, osteoarthritis, and other inflammatory joint diseases

Kinne, Raimund W; Liehr, Thomas; Beensen, Volkmar; Kunisch, Elke; Zimmermann, Thomas; Holland, Heidrun; Pfeiffer, Robert; Stahl, Hans-Detlev; Lungershausen, Wolfgang; Hein, Gert; Roth, Andreas; Emmrich, Frank; Claussen, Uwe; Froster, Ursula G

doi:10.1186/ar322

Table 1 Cytogenetic findings in various cells/nuclei from patients with rheumatoid arthritis

From: Mosaic chromosomal aberrations in synovial fibroblasts of patients with rheumatoid arthritis, osteoarthritis, and other inflammatory joint diseases

	Karyotype and FISH results^a
	Peripheral blood lymphocytes	Nuclei extracted from synovial tissue^b	Collagenase digest	Synovial fibroblasts
Patient				P-1	P-4
ES43	46,XX	n.a.	n.a.	46,XX	n.a.
ES51	46,XX	n.a.	n.a.	47,XX,+7[9]/46,XX [41]	n.a.
ES79	46,XY	n.a.	n.a.	46,XY	n.a.
L07	46,XY	n.a.	n.a.	46,XY	n.a.
L12	46,XY	n.a.	n.a.	46,XY	n.a.
VS01	46,XY	n.a.	n.a.	46,XY	n.a.
AA5	n.a.	n.a.	n.a.	n.a.	n.a.
				FISH:+6 [23%];+7 [19%];+8 [17%]; +9 [18%];incl. polyploid [11%]
EB1	46,XY	n.a.	n.a.	46,XY	n.a.
EB2	46,XX	n.a.	n.a.	46,XX	n.a.
					FISH:+6 [16%];+7 [31%];+8 [22%]; +9 [20%];incl. polyploid [14%]
EB4	46,XX	n.a.	n.a.	n.a.	46,XX
					FISH:+7 [33%];+8 [12%]; -9 [15%];+18 [9%];-18 [11%]; incl. polyploid [4%]
EB5	46,XY	n.a.	n.a.	46,XY,del(15)(q22),-21,+mar [cp3]/46,XY[2]	46,XY (Passage 6)
				FISH:+6 [5%];+7 [11%];-8 [8%]; +9 [6%];incl. polyploid [2%]	FISH: +7 [6%];+8 [10%];+9 [6%]; incl. polyploid [4%]
EB6	46,XX		n.a.	47,XX,+76;46,XX[6]	n.a.
		FISH:+6 [7%];+7 [12%]; +8 [2%];-8 [0%];-18 [8%]; incl. polyploid [1%]		FISH:+6 [3%];+7 [40%];+8 [5%]; -8 [8%];-18 [9%]	FISH:+6 [13%];+7 [58%];+8 [8%]; -8 [4%];-18 [84%]; incl. polyploid [2%]
EB7	n.a.		n.a.	46,XY (1/12 metaphases 45,X,-Y	46,XY (1/10 metaphases 45,X,-Y,+7?)
		FISH:+6 [6%];+7 [3%]; -18 [9%];+/-Y [n.a.]; incl. polyploid [1%]		FISH:+6 [n.a.];+7 [5%];-18 [8%]; -Y [5%];+Y [4%]; incl. polyploid [2%]	FISH: :+6 [n.a.];+7 [4%];-18 [n.a.]; -Y [30%];+Y [0%]
EB20	46,XY		46,XY	46,XY	n.a.
	FISH: normal	FISH:+7 [2%];+12 [5%]	FISH:+7 [4%]; +12 [5%]	FISH:+7 [9%];+12 [14%]; incl. polyploid [1%]
EB23	46,XX	n.a.	46,XX	46,XX	46,XX
	FISH: normal		FISH:+7 [4%]; +9 [3%];+18 [1%]	FISH:+7 [9%];+9 [5%];+18 [2%]	FISH:+7 [13%];+9 [11%];+18 [7%]
EB25	46,XX		n.a.	46,XX	n.a.
	FISH: normal	FISH:+8 [5%]		FISH: normal
EB26	46,XY		46,XY	46,XY	47,XY,+74/46,XY[4]
	FISH: normal	FISH:+6 [2%];+7 [8%]; +8 [n.a.];+9 [3%]	FISH:+6 [0%]; +7 [12%];+8 [3%]; +9 [0%]	FISH:+6 [3%];+7 [14%];+8 [5%]; +9 [2%];incl. polyploid [2%]	FISH:+6 [10%];+7 [9%];+8 [n.a.]; +9 [9%];incl. polyploid [1%]
EB27	46,XX		n.a.	47,XX,+7[2]	n.a.
	FISH: normal	FISH:+7 [0%]; +8 [8%];+18 [7%]	FISH:+7 [7%]; +8 [n.a.];+18 [n.a.]	FISH:+7 [13%];+8 [n.a.];+18 [n.a.] incl. polyploid [2%]	FISH:+7 [20%];+8 [n.a.];+18 [n.a.] incl. polyploid [4%]
EB28	46,XY		n.a.	n.a.	n.a.
	FISH: normal	FISH:+4 [8%];+7 [12%]
EB30	46,XX	n.a.	n.a.	n.a.	n.a.
	FISH: normal
EB31	46,XX,16qh+	n.a.	46,XX,16qh+	46,XX,16qh+	n.a.
	FISH: normal		FISH:+4 [1%]; +7 [4%]	FISH:+4 [5%];+7 [5%]; incl. polyploid [5%]

^aData sets are not complete for all patients due to limited sample availability and the paucity of mitoses in some cell cultures. ^bOnly FISH data are available. incl. = including; n.a. = not analyzed (Karyotype and/or FISH); P-1 = passage 1; P-4 = passage 4.

Back to article page

ISSN: 1478-6362

Contact us

General enquiries: journalsubmissions@springernature.com

Arthritis Research & Therapy

Contact us