Figure 4

Effect of interleukin (IL)-17 on the production of macrophage colony-stimulating factor (M-CSF), tumor necrosis factor α (TNF-α) and IL-6. Synovial tissue (ST)-derived inflammatory cells were incubated with incremental doses of IL-17 in the absence or presence of indomethacin (100 to 1,000 nM) for 1 week. Enzyme-linked immunosorbent assay kits were used to measure the concentration of (A) TNF-α, (B) M-CSF and IL-6 (C) in the culture supernatants derived from seven donors. There were no significant differences in the production of IL-6 between the presence and absence of indomethacin. Box and whisker plots indicate 25th/75th percentile and minimum/maximum, respectively. Bar indicates the median value. *P < 0.05 (by Wilcoxon signed-rank test).