Table 1 Primer sequences for the present study
Primer | Sequence (5' to 3') | |
|---|---|---|
Sense | Antisense | |
TβRI | TTAAAAGGCGCAACCAAGAAC | GTGGTGATGAGCCCTTCGAT |
TβRII | GACATCAATCTGAAGCATGAGAACA | GGCGGTGATCAGCCAGTATT |
Smad2 | GCTGTTTTCCTAGCGTGGCTT | TCCAGACCCACCAGCTGACT |
Smad3 | GCATCAGCCGCTTCTCAAGT | ATCTCCCCACCATCACCTCC |
Smad4 | CCTTCTGGAGGAGATCGCT | TCAATGGCTTCTGTCCTGTGG |
Smad7 | AATGTGTTTTCTAGATTCCCAACTTCTT | CACTCTCGTCTTCTCCTCCCAGTA |
Sp1 | AGAATTGAGTCACCCAATGAGAACA | GTTGTGTGGCTGTGAGGTCAAG |
COL2A1 | GGCAATAGCAGGTTCACGTACA | CGATAACAGTCTTGCCCCACTT |
COL1A1 | CACCAATCACCTGCGGTACAGAA | CAGATCACGTCATCGCACAAC |
COL10A1 | CCTGGTATGAATGGACAGAAAGG | CCCTGAGGGCCTGGAAGA |
Aggrecan | TCGAGGACAGCGAGGCC | TCGAGGGTGTAGCGTGTAGAGA |
Sox9 | CCC ATG TGG AAG GCA GAT G | TTC TGA GAG GCA CAG GTG ACA |