Pam3CSK4 induced IL-6 and IL-8 in mononuclear cells from RA patients. SFMCs (A; n = 6) and PBMCs (B; n = 11) were stimulated with TLR2 agonist Pam3CSK4 at 200 ng/ml. Levels of IL-6 and IL-8 in the culture supernatants were determined and compared to unstimulated cells (Basal) at six hours. Values are expressed as the mean ± SEM. * P < 0.05, significantly different from control as determined using Wilcoxon Signed Rank analysis.