Expression of TIM-2 on CD4 T and B cells. (a) Expression of T cell immunoglobulin and mucin domain (TIM)-2 on activated CD4 T cells. Purified splenic CD4 T cells were stimulated by immobilized anti-CD3 monoclonal antibody (mAb) with or without anti-CD28 mAb and harvested at the indicated periods. Cells were stained with biotinylated RMT2-26 or control IgG followed by PE-labeled streptavidin. (b) Expression of TIM-2 on activated B cells. Purified splenic B cells were stimulated with the indicated combinations of anti-IgM Ab, anti-CD40 mAb, and IL-4. Cells were harvested at the indicated periods and stained with biotinylated RMT2-26 or control IgG followed by PE-labeled streptavidin. Thick lines indicate the staining with anti-TIM-2 mAb and the dotted lines indicate background staining with control IgG.