Effect of anti-TIM-2 mAbs on B cell proliferation in vitro. Purified splenic B cells from (a) DBA/1, (b) BALB/c, or (c) FcRγ-deficient mice were stimulated with anti-IgM, anti-CD40, and IL-4 in the absence or presence of H-ferritin and indicated monoclonal antibodies (mAbs) for 48 hours. Proliferative response was assessed by pulsing the cultures with 0.5 μCi/well 3H-thymidine for the last six hours. Data are expressed as the mean ± standard error of the mean of triplicate wells. *, P < 0.05; **, P < 0.01 as compared with control IgG. The results are representative of two experiments in each mouse strain. TIM, T cell immunoglobulin and mucin domain.