Figure 4From: TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritisTWEAK expression by PBMC. PBMC from two healthy volunteers were sorted by FACS based on their expression of CD22, yielding CD22+ and CD22- populations of greater than 94% purity based on post-sort analysis (A). Isolated cells were then analysed for TWEAK mRNA expression relative to that of GAPDH, by real-time RT-PCR (B). Data shown are means of triplicate reactions ± SD. Differences in relative expression of TWEAK mRNA between CD22+ and CD22- populations were tested by Student's t-test (**P < 0.001).Back to article page