Cystatin C-deficient DCs have increased expression of MHCII and Co-stimulatory molecules, and more activated T cells. Splenocytes from naïve mice or splenocytes stimulated with ConA or media were analyzed by flow cytometry. Naive DCs from cystatin C-deficient (CysC-/-) mice expressed higher intensity of MHCII (A) CD80 (B) and CD86 (C). A greater percent of CD4+ T cells expressed CD69 in cystatin C-deficient mice than wild type mice (CysC+/+) when stimulated with ConA for 24 h (D) but not CD8+ T cells. (E), In line with this there was a higher percent of CD4+ T cells in cystatin C-deficient mice and a tendency for a higher percent of CD8+ and total T cells (F). Six mice were used per group, statistical significance was calculated by Students t-test where * P-value < 0.05 and ** P-value < 0.01.