From: Regulation of chondrocyte gene expression by osteogenic protein-1
Primer | Orientation | Sequence | Annealing temp and time | Accession no. |
---|---|---|---|---|
18SrRNA |  | Qiagen QuantiTect Primer Assay | 62°C, 40 sec | [GenBank:X03205] |
β-actin | Forward | 5'-TCCATCATGAAGTGTGACGTGGAC-3' | 62°C, 40 sec | [GenBank:NM_001101] |
 | Reverse | 5'-TTGATCTTCATTGTGCTGGGTGCC-3' |  |  |
BMP-2 |  | Qiagen QuantiTect Primer Assay | 60°C, 40 sec | [GenBank::NM_001200] |
GAPDH | Forward | 5'-TGGACTCCACGACGTACTCAG-3' | 62°C, 40 sec | [GenBank:NM_002046] |
 | Reverse | 5'-CGGGAAGCTTGTCATCAATGGAA-3' |  |  |
Gremlin-1 | Forward | 5'-ATACCTGAAGCGAGACTGGTGCAA-3' | 64°C, 40 sec | [GenBank:NM_013372] |
 | Reverse | 5'-AACAGAAGCGGTTGATGATGGTGC-3' |  |  |
IL-6 | Forward | 5'-GTCAATTCGTTCTGAAGAGGTGAGT-3' | 64°C, 40 sec | [GenBank:NM_000600] |
 | Reverse | 5'-CCCCAGGAGAAGATTCCAAAGATG-3' |  |  |
IL-8 | Forward | 5'-AGACATACTCCAAACCTTTCCACCC-3' | 58°C, 30 sec | [GenBank:NM_000584] |
 | Reverse | 5'-ATTTCTGTGTTGGCGCAGTGTGGT-3' |  |  |
LIF-1 | Forward | 5'-TAAGGAGGCCTCGCAGGATGTC-3' | 64°C, 30 sec | [GenBank:NM_002309] |
 | Reverse | 5'-TAGTCGTGTACCTTGGCACCTC-3' |  |  |