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Table 1 Sequence of primers for quantitative real time PCR

From: Regulation of chondrocyte gene expression by osteogenic protein-1

Primer Orientation Sequence Annealing temp and time Accession no.
18SrRNA   Qiagen QuantiTect Primer Assay 62°C, 40 sec [GenBank:X03205]
β-actin Forward 5'-TCCATCATGAAGTGTGACGTGGAC-3' 62°C, 40 sec [GenBank:NM_001101]
  Reverse 5'-TTGATCTTCATTGTGCTGGGTGCC-3'   
BMP-2   Qiagen QuantiTect Primer Assay 60°C, 40 sec [GenBank::NM_001200]
GAPDH Forward 5'-TGGACTCCACGACGTACTCAG-3' 62°C, 40 sec [GenBank:NM_002046]
  Reverse 5'-CGGGAAGCTTGTCATCAATGGAA-3'   
Gremlin-1 Forward 5'-ATACCTGAAGCGAGACTGGTGCAA-3' 64°C, 40 sec [GenBank:NM_013372]
  Reverse 5'-AACAGAAGCGGTTGATGATGGTGC-3'   
IL-6 Forward 5'-GTCAATTCGTTCTGAAGAGGTGAGT-3' 64°C, 40 sec [GenBank:NM_000600]
  Reverse 5'-CCCCAGGAGAAGATTCCAAAGATG-3'   
IL-8 Forward 5'-AGACATACTCCAAACCTTTCCACCC-3' 58°C, 30 sec [GenBank:NM_000584]
  Reverse 5'-ATTTCTGTGTTGGCGCAGTGTGGT-3'   
LIF-1 Forward 5'-TAAGGAGGCCTCGCAGGATGTC-3' 64°C, 30 sec [GenBank:NM_002309]
  Reverse 5'-TAGTCGTGTACCTTGGCACCTC-3'