Early gene expression in IL-1-treated SW1353 chondrocytes. SW1353 cells were stimulated for the indicated times with IL-1β (10 ng/ml) and total RNA was isolated. RNA was treated with DNAse I and then reverse transcribed with Moloney murine leukemia virus reverse transcriptase and oligodT primers. A tenth of the reverse transcriptase reaction was amplified using gene-specific primers, Platinum Taq DNA polymerase, and 33PdATP. Single-band products were resolved on native acrylamide gels and visualized by autoradiography.