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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage

Figure 2

FACS analysis of CD105 and CD166 expressions on isolated normal and osteoarthritis (OA) chondrocytes and enrichment of CD166+OA cells after immunomagnetic separation. Enzymatically isolated, non-separated OA (n = 11 patients) and normal (n = 3) chondrocytes were subjected to FACS analysis after initial in vitro culture (a). After magnetic separation of CD166+ cells, both the positive (b) and the negative (c) fractions were studied for the expressions of CD105 and CD166. The bar charts show the mean ± standard error of the mean (SEM) for all analyzed patients; for each section, a representative example of the FACS measurement is included as a dot plot diagram, in which CD105+/CD166+ cells are located in the upper right quadrant. In addition, the relative expressions of CD105 and CD166 (colored lines) and the corresponding isotype controls (filled graphs) are shown in histograms. FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; RPE, R-phycoerythrin.

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