Figure 5

Suppression of carrageenan-induced inflammation by CEP-33779. Balb/c mice were prepared for air pouch initiation (see Materials and methods) using sterile air. Carrageenan at 1% in saline solution was injected directly into the pouch, followed immediately by CEP-33779 or Dex treatment 1 hour later. One hundred microliters of air pouch fluid were removed 24 hours after compound administration. (A) Total cell count using 7-aminoactinomycin D exclusion via flow cytometeric analysis and BD TruCount beads. (B) Fluid supernatant IL-6 concentration as determined by Luminex cytokine analysis. (C) Hemoglobin content was assessed by the Drabkin's assay. All graphs show means ± SEM. *P < 0.05 with two-way ANOVA used as the statistical test; n = 5 mice per group tested.