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Figure 3 | Arthritis Research & Therapy

Figure 3

From: CP690,550 inhibits oncostatin M-induced JAK/STAT signaling pathway in rheumatoid synoviocytes

Figure 3

CP690,550 suppresses OSM-induced IL-6 synthesis in RA-FLS; CP690,550 suppresses OSM-induced IL-6 mRNA expression in RA-FLS. (a) RA-FLS were pretreated with vehicle (DMSO, -) or indicated concentrations of CP690,550 for two hours. Cells were stimulated with OSM (20 ng/ml) for 24 hours, after which conditioned media were collected and IL-6 content was measured by ELISA. The data were expressed as the mean ± SD of three independent experiments. *P < 0.05 compared to OSM-treated RA-FLS. **P < 0.01 compared to OSM-treated RA-FLS. (b) RA-FLS were pretreated with vehicle (DMSO, -) or indicated concentrations of CP690,550 for two hours. Cells were stimulated with OSM (20 ng/ml) for four hours, after which, IL-6 and GAPDH mRNA expression was determined by real-time PCR method. The data were expressed as the mean ± SD of three independent experiments. *P < 0.01 compared to OSM-treated RA-FLS. **P < 0.001 compared to OSM-treated RA-FLS.

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