Figure 4
From: CP690,550 inhibits oncostatin M-induced JAK/STAT signaling pathway in rheumatoid synoviocytes
Phosphorylation of JAKs/STATs in OSM-treated RA-FLS. Quiescent RA-FLS were stimulated with OSM (20 ng/ml) for indicated times. Phosphorylation of JAKs (A; JAK1, JAK2, JAK3) and STATs (B; STAT1, STAT3, STAT5) were determined by Western blotting using phospho-specific or pan antibodies against JAK1, JAK2, JAK3, STAT1, STAT3 and STAT5. Three experiments were performed using different RA-FLS and a representative result is shown.