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Table 3 Proteins specifically recognised by IgG from at least four pools of patients with unidentified ANA

From: Identification of new autoantibody specificities directed at proteins involved in the transforming growth factor β pathway in patients with systemic sclerosis

Protein ID on gel HEp-2 cell protein SwissProt accession number MW th/es pHi th/es Number of unique identified peptides# Total ion score Best ion score Sequence coverage (%)
550 Far upstream element-binding protein 2 (N) [SwissProt:FUBP2_HUMAN] 73/80 6.8/7.1 10/17 554 108 37
553 Far upstream element-binding protein 2 (N) [SwissProt:FUBP2_HUMAN] 73/79 6.8/7.3 11/17 864 153 32
554 Far upstream element-binding protein 2 (N) [SwissProt:FUBP2_HUMAN] 73/79 6.8/7.5 10/17 598 105 34
617 Lamin A/C (N) [SwissProt:LMNA_HUMAN] 74/73 6.6/7.0 11/29 680 127 50
762 RNA-binding protein FUS (N) [SwissProt:FUS_HUMAN] 53/61 9.4/7.8 2/5 64 45 17
771 Ras GTPase-activating protein-binding protein 1 (N) [SwissProt:G3BP1_HUMAN] 52/61 5.4/6.0 5/12 381 131 39
913 Lamin A/C (T) [SwissProt:LMNA_HUMAN] 74/77 6.6/7.0 5/14 120 39 28
914 Lamin A/C (T) [SwissProt:LMNA_HUMAN] 74/77 6.6/6.8 7/23 121 38 42
921 RuvB-like 1 (N) [SwissProt:RUVB1_HUMAN] 50/50 6.0/6.8 8/16 591 131 50
  Protein DEK (N) [SwissProt:DEK_HUMAN] 43/50 8.7/6.8 2/4 162 92 12
924 Heterogeneous nuclear ribonucleoprotein H (N) [SwissProt:HNRH1_HUMAN] 49/49 5.9/6.4 8/15 440 80 53
1132 60-kDa heat shock protein, mitochondrial precursor (T) [SwissProt:CH60_HUMAN] 61/61 5.7/5.5 7/15 176 36 28
1191 Serine/threonine protein phosphatase PP1-β catalytic subunit (N) [SwissProt:PP1B_HUMAN] 37/34 5.8/6.1 2/10 62 41 35
1629 Annexin A1 (T) [SwissProt:ANXA1_HUMAN] 39/38 6.6/6.7 6/13 233 73 50
2212 Stathmin (T) [SwissProt:STMN1_HUMAN] 17/18 5.8/6.2 2/6 82 51 32
2039 Histone-binding protein RBBP4 (N) [SwissProt:RBBP4_HUMAN] 48/48 4.7/5.1 7/10 414 103 27
  1. aANA: antinuclear antibody; FUS: fused in sarcoma; MW: molecular weight (in kilodaltons); N: proteins recognised in HEp-2 cell-enriched nuclear protein extract; pHi, intracellular pH; PP1: protein phosphatase 1; SSc: systemic sclerosis; T: proteins recognised in HEp-2 cell total protein extract; th/es: theoretical/estimated. bNumber of uniquely identified peptides in tandem mass spectrometry (MS/MS) and mass spectrometry + MS/MS searches.