Production of IL-17A and expression of Foxp3 by inguinal lymph node cells of SKG mice. (A) Inguinal lymph node cells of SKG mice in each group were collected on Day 35. Cells were stimulated with phorbol myristate acetate/ionomycin and the supernatants were collected after 8 h for the measurement of IL-17A. Values are presented as the mean ± SE (DMSO group: n = 3, TSA group: n = 3, P < 0.0001). (B) The expression of Foxp3 in inguinal lymph node cells in SKG mice. Inguinal lymph node cells of SKG mice were collected on Day 35 in each group as previously described. Cells were stained for anti-CD4, anti-CD25, and Foxp3. The percentage of CD4+CD25+Foxp3+ cells among gated CD4+ cells was determined. Results are expressed as the mean ± SE (DMSO group: n = 4, TSA group: n = 4, P = 0.038).