Figure 1

Grey-level intensity in entire calvariae, as assessed by 3D micro-CT. In the absence of PE particles, mean grey-level values decreased in ovariectomized (OVX) mice as compared to normal controls (NC) and Sham-OVX mice. Of note is the significant increase of grey-level intensity in calvarial bone tissue of OVX animals supplemented with oestrogen (E), as compared to OVX mice. Grey-level values were obtained using CTAn software. The data are presented as means ± standard error of the mean (a indicates P < 0.05, as compared to OVX mice, using the Mann-Whitney U test).