Effect of Gα
siRNA (small interfering RNA) on CXCL10-induced expression of receptor activator of nuclear factor kappa-B ligand (RANKL). Jurkat T cells were transfected with Gαi2 and control siRNA by electroporation. After 48 hours, the cells were treated with 10 ng/mL CXCL10 for 6 hours, and then RANKL expression was assessed by immunoblot analysis using a specific antibody against RANKL (a). The bolts shown are representative of at least four independent experiments, and the histograms show average and standard deviations of representative RANKL expression (b). CXCL10, interferon-gamma-inducible protein 10.