Differential CD3+CD4+T cell subset distribution between healthy controls and systemic lupus erythematosus patients Distribution of CD45RA- CD45RO+ (RA- RO+) memory T cells and CD45RA+ CD45RO- (RA+ RO-) naïve T cells (a), or CD45RO- (RO-) CD62L+ naïve T cells, CD45RO+ (RO+) CD62L+ central memory T cells and CD45RO+ (RO+) CD62L- effector memory T cells in the peripheral blood mononuclear cells (PBMCs) of SLE patients and HCs (b). (c) CD4+ T cells activated for three days with anti-CD3 and anti-CD28 were compared between patients and HCs according to their CD25 and FOXP3 expression. (d) Percentage of expanded CD25+ regulatory T cells (Tregs) in SLE patients according to their disease activity. (e) The percentage of CD25- Tregs was positively correlated with the percentage of memory CD45RO+ CD45RA- CD4+ T cells in HCs but not in patients. (f) The percentage of CD25+ Tregs was negatively correlated (one-tail P-value) with the percentage of memory CD45RO+ CD45RA- CD4+ T cells in HCs but not in patients. The graphs in (e-f) show the linear regression lines for HCs (dashed) and SLE patients (plain), the P-values for the Spearman correlation tests and the R2 values calculated separately for the patient and HC cohorts. Ns, non-significant.