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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Defective response of CD4+T cells to retinoic acid and TGFβ in systemic lupus erythematosus

Figure 3

Representative fluorescence activated cell sorter (FACS) plots showing the regulatory T cell (Treg) populations used in the suppression assays (a) Standardized cord blood Treg used as positive controls, the great majority of which being CD127- CD25+ FOXP3+. (b) Treg isolated from a systemic lupus erythematosus (SLE) patient as CD4+ CD127-, then sorted as CD25+ or CD25- after stimulation and expansion with transforming growth factor beta (TGFβ) and retinoic acid (RA). The CD25+-sorted population was approximately 80% FoxP3+ CD25+, while the CD25--sorted population was more than 80% FoxP3+ CD25-. (c) Proliferation of CD25+ and CD25- Treg isolated from a same patient in the presence of standardized peripheral blood mononuclear cells (PBMCs) at the same dilution (1:4), in the presence of anti-CD3 and anti-CD28 for six days, showing a robust response of the CD25+ as opposed to the CD25- Tregs.

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