Table 2 Mass spectrometry data of the endothelial cell protein spots identified as specific target antigensa
Spot ID | Protein | SwissProt accession number | Theoretical/estimated MW, kDa | Theoretical/estimated pI | Number of unique identified peptidesc | Total ion score | Best ion score | Sequence coverage, % |
|---|---|---|---|---|---|---|---|---|
228 | Vinculin | [Swiss Prot:VINC_HUMAN] | 124/116 | 5.5/6.6 | 3/13 | 53 | 34 | 15 |
461 | Lamin A/C | [Swiss Prot:LMNA_HUMAN] | 74/80 | 6.6/7.3 | 11/23 | 573 | 82 | 39 |
Semaphorin-4D precursor | [Swiss Prot:SEM4D_HUMAN] | 96/80 | 8.3/7.3 | 2/2 | 44 | 29 | 2 | |
476 | Ezrin | [Swiss Prot:EZRI_HUMAN] | 69/79 | 5.9/7.0 | 2/8 | 92 | 58 | 13 |
Moesin | [Swiss Prot:MOES_HUMAN] | 67/79 | 6.1/7.0 | 3/12 | 186 | 96 | 19 | |
Lamin A/C | [Swiss Prot:LMNA_HUMAN] | 74/79 | 6.6/7.0 | 8/21 | 314 | 70 | 37 | |
Radixin | [Swiss Prot: RADI_HUMAN] | 68/79 | 6.0/7.0 | 2/6 | 92 | 58 | 10 | |
Semaphorin-4D precursor | [Swiss Prot:SEM4D_HUMAN] | 96/79 | 8.3/7.0 | 2/2 | 35 | 20 | 2 | |
557 | Far upstream element-binding protein 1 | [Swiss Prot:FUBP1_HUMAN] | 67/75 | 7.2/7.2 | 3/7 | 114 | 47 | 13 |
631 | Lamin A/C | [Swiss Prot:LMNA_HUMAN] | 74/71 | 6.6/6.9 | 6/10 | 184 | 48 | 15 |
646 | Lamin A/C | [Swiss Prot:LMNA_HUMAN] | 74/70 | 6.6/7.0 | 12/28 | 482 | 71 | 46 |
680 | No protein identified | /66 | /8.0 | |||||
681 | No protein identified | /66 | /8.2 | |||||
683 | No protein identified | /66 | /8.6 | |||||
703 | No protein identified | /65 | /5.9 | |||||
768 | No protein identified | /60 | /7.9 | |||||
784 | Dihydrolipoyl dehydrogenase, mitochondrial precursor | [Swiss Prot:DLDH_HUMAN] | 54/59 | 7.6/7.3 | 2/2 | 42 | 22 | 5 |
789 | Inosine 5'-monophosphate dehydrogenase 2 | [Swiss Prot:IMDH2_HUMAN] | 56/58 | 6.4/7.1 | 4/7 | 169 | 94 | 17 |
853 | No protein identified | /54 | /6 | |||||
908 | α-enolase | [Swiss Prot:ENOA_HUMAN] | 47/50 | 7.0/8.3 | 7/12 | 450 | 143 | 47 |
950 | Tripeptidyl peptidase 1 precursor | [Swiss Prot:TPP1_HUMAN] | 61/50 | 6.0/6.4 | 3/5 | 89 | 34 | 15 |
1017 | Fumarate hydratase, mitochondrial precursor | [Swiss Prot:FUMH_HUMAN] | 55/48 | 8.9/8.0 | 6/7 | 243 | 71 | 24 |
1085 | Heterogeneous nuclear ribonucleoprotein D0 | [Swiss Prot:HNRPD_HUMAN] | 38/43 | 7.6/7.8 | 3/3 | 122 | 69 | 11 |
1214 | PDZ and LIM domain protein 1 | [Swiss Prot:PDLI1_HUMAN] | 36/37 | 6.6/7.4 | 5/10 | 269 | 62 | 44 |
1249 | 60S acidic ribosomal protein P0 | [Swiss Prot:RLA0_HUMAN] | 34/37 | 5.7/6.0 | 2/5 | 56 | 35 | 21 |
1352 | Voltage-dependent anion-selective channel protein 2 | [Swiss Prot:VDAC2_HUMAN] | 32/33 | 7.5/7.4 | 4/4 | 155 | 75 | 18 |
1359 | Annexin A5 | [Swiss Prot:ANXA5_HUMAN] | 36/33 | 4.9/5.3 | 10/12 | 538 | 86 | 52 |
1376 | No protein identified | /32 | /7.5 | |||||
1440 | Heat shock protein β1 | [Swiss Prot:HSPB1_HUMAN] | 23/29 | 6.0/6.2 | 5/8 | 382 | 138 | 47 |
NADH dehydrogenase [ubiquinone] iron-sulphur protein 3, mitochondrial precursor | [Swiss Prot:NDUS3_HUMAN] | 30/29 | 7.0/6.2 | 2/6 | 70 | 38 | 26 | |
1614 | Protein DJ-1 | [Swiss Prot:PARK7_HUMAN] | 20/25 | 6.3/6.6 | 5/5 | 202 | 75 | 51 |
1632 | No protein identified | /23 | /5.3 | |||||
1734 | Peptidyl-prolyl cis-trans isomerase A | [Swiss Prot:PPIA_HUMAN] | 18/18 | 7.7/8 | 3/5 | 78 | 48 | 36 |
1817 | Thioredoxin-dependent peroxide reductase, mitochondrial precursor | [Swiss Prot:PRDX3_HUMAN] | 28/15 | 7.7/6.8 | 5/6 | 211 | 61 | 44 |
1821 | Fatty acid-binding protein, epidermalb | [Swiss Prot:FABP5_HUMAN] | 15/15 | 6.6/6.7 | 1/5 | 26 | 26 | 47 |
2120 | Elongation factor Tu, mitochondrial precursor | [Swiss Prot:EFTU_HUMAN] | 50/45 | 7.3/6.9 | 5/6 | 120 | 58 | 15 |
Poly(rC)-binding protein 1b | [Swiss Prot:PCBP1_HUMAN] | 37/46 | 6.7/6.9 | 1/2 | 36 | 36 | 6 | |
Heterogeneous nuclear ribonucleoprotein D0b | [Swiss Prot:HNRPD_HUMAN] | 38/45 | 7.6/6.9 | 1/4 | 39 | 36 | 10 |