Figure 3
Dose-response relation for NASHA regarding antinociceptive effects on different days. (a to d) Increase in thresholds in relation to saline (0%) and morphine (100%) on days 1, 7, 14, and 21 after injection. Overall, only the higher doses (50, 75, and 100 μl, each n = 11) show an antinociceptive effect of more than 50% beyond day 1, but not 10 and 30 μl (n = 11 and n = 10, respectively). Fitting of linear and sigmoid curves (only linear fitting was possible for day 14) revealed no clear-cut relation, but apparently a certain threshold dose is needed to obtain antinociceptive effects. Data are presented as mean ± standard error of the mean.