Figure 3

IL-17 and IL-1β increased RAGE mRNA expression in RA-FLS. (a) RA-FLS were cultured with 10 ng/ml IL-17, 5 ng/ml TNF-α, and 1 ng/ml IL-1β for 24 h, and RAGE mRNA was analyzed by real-time PCR. The lactate dehydrogenase (LDH) concentrations in the culture supernatants were determined by an activity assay kit. (b) RA-FLS were cultured as in Figure 3a. RAGE expression in the FLS was determined using a RAGE-specific antibody. The brown color shows the RAGE. (c) RAGE protein expression was identified by Western blot. Values are the mean ± SEM of triplicate cultures. *P < 0.05 compared to untreated cells and #P < 0.05 compared to IL-17-treated cells.