Figure 6

Cellular composition of lymphoid organs in IIJ mice. (A) and (B) Average number of cells of specific immune lineages in (A) lymph nodes and (B) spleens of AR or NAR IIJ mice based on flow cytometric analysis. Error bars represent SEM. Live cells were gated on and the amount of each cell type (CD3⁺ = T cell, B220⁺ = B cell and CD11b⁺ = myeloid/granulocyte) was determined as the percentage of CD45⁺ cells. All AR mice were more than seven weeks old and had been arthritic for a minimum of four weeks. NAR mice were littermates or age-matched within two weeks (n = 4 to 5 mice/group). Groups were compared by two-way analysis of variance, and cellular composition varied significantly in the lymph nodes (P < 0.05) but not in the spleens.