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Figure 4 | Arthritis Research & Therapy

Figure 4

From: The Bruton tyrosine kinase inhibitor PCI-32765 ameliorates autoimmune arthritis by inhibition of multiple effector cells

Figure 4

PCI-32765 inhibits FcγR signaling in monocytes. (a) Inhibition of Btk signaling in THP-1 cells. THP-1 cells were stimulated with IFN-γ and pretreated with PCI-32765 (1 μM or 0.1 μM) or vehicle and stimulated by cross-linking with human IgG and goat anti-human IgG in the presence of H2O2 and IFNγ. Cell lysates were subjected to western blot analysis and probed with the indicated antibodies. (b) Inhibition of calcium mobilization in THP-1 cells (upper panel) or human monocytes (lower panel) stimulated with cross-linked IgG as described in Materials and Methods.

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