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Table 5 Comparison of the performance characteristics of ELISA and MLDAf

From: Single-step autoantibody profiling in antiphospholipid syndrome using a multi-line dot assay

  Sensitivity 95% CI specificity 95% CI +LR 95% CI -LR 95% CI
at least one aPL antibody by ELISA 58.8 47.6 to 69.4 95.8 91.1 to 98.5 14.1 6.3 to 31.5 0.4 0.3 to 0.6
at least one aPL antibody by MLDA 67.9 56.8 to 77.6 89.6 83.4 to 94.0 6.5 4.0 to 10.8 0.4 0.3 to 0.5
at least one aPL IgG or at least three aPL IgM by MLDA 67.1 56.0 to 76.9 96.5 92.1 to 98.9 19.3 8.1 to 46.3 0.3 0.2 to 0.5
  1. Sensitivity, specificity and likelihood ratios were calculated using a cut-off of 10 U/ml for all ELISA investigating 85 APS patients, 65 DC patients, and 79 NHS.
  2. anti-β2 GPI, anti-beta2-glycoprotein I; anti-CL, anti-cardiolipin; aPL, anti-phospholipid antibody; ELISA, enzyme-linked immunosorbent immunoassay; MLDA, multi-line dot assay; +LR, positive likelihood ratio; -LR, negative likelihood ratio.