Table 2 Oligonucleotide primers used to assess gene expression
From: The efficacy of Link N as a mediator of repair in a rabbit model of intervertebral disc degeneration
Gene | Primer sequence (5' to 3') | Annealing temperature (°C) |
|---|---|---|
Aggrecan | Forward: GAGGTCGTGGTGAAAGGTGT | 60 |
| Â | Reverse: GTGTGGATGGGGTACCTGAC | Â |
COL1A1 | Forward: AGGGCCAAGACGAAGACATC | 62 |
| Â | Reverse: AGATCACGTCATCGCACAACA | Â |
COL2A1 | Forward: CAACACTGCCAACGTCCAGAT | 62 |
| Â | Reverse: CTGCTTCGTCCAGATAGGCAAT | Â |
MMP-3 | Forward: TTTTGGCCATCTCTTCCTTCA | 65 |
| Â | Reverse: TGTGGATGCCTCTGGGTATC | Â |
ADAMTS-4 | Forward: GACCTTCCGTGAAGAGCAGTGT | 58 |
| Â | Reverse: CCTGGCAGGTGAGTTTGCAT | Â |
ADAMTS-5 | Forward: CCTGGCAGGTGAGTTTGCAT | 60 |
| Â | Reverse: GGAGAACATATGGTCCCAACGT | Â |
GAPDH | Forward: ACTCTGGCAAAGTGGATG | 60 |
| Â | Reverse: TCCTGGAAGATGGTGATG | Â |