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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Decreased catalytic function with altered sumoylation of DNA topoisomerase I in the nuclei of scleroderma fibroblasts

Figure 2

Comparison of topo I staining in cultured fibroblasts of normal controls and SSc patients. A. Topo I immunostaining with anti-topo I monoclonal antibodies showed multiple speckles in the nucleoplasm of SSc fibroblasts, which is differentiated from that in normal fibroblasts (relatively homogenous stain of topo I) at both 7 and 14 days of cultures. Some SSc fibroblasts show cytoplasmic staining of topo I protein (marked with red arrow heads). B. Topo I immunostaining with anti-topo I positive sera from SSc patients show the expected nuclear/nucleolar staining as well as cytoplasmic staining of SSc fibroblasts. At Day 14, the cytoplasmic staining appeared to increase relative to the nucleoplasm and nucleolar staining.

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