Monitoring IL-1β-induced MMP-3 and chemokine production on pretreatment with IDR-1002. Human fibroblast-like synoviocytes (FLS) were pretreated with IDR-1002 for 45 minutes before stimulation with IL-1β (10 ng/ml) for 24 hours. Tissue-culture supernatants were monitored for (a) MMP-3 production with ELISA, and chemokines, (b) MCP-1, and (c), IL-8 production by using the BDCytometric Bead Array (CBA) preconfigured human chemokine multiplex system with FACS Caliburflow cytometer (BD Biosciences). Results shown are an average of three independent biologic experiments performed with cells isolated from synovial tissues of three different donors ± standard error (*P < 0.05, **P < 0.01). IDR, innate defence regulator; IL, interleukin; MMP-3, matrix metalloproteinase-3.