Fibroblast growth factor receptor 1 is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular chondrocytes

Table 1 Real-time PCR primer sequences

Gene	Primer Sequence (5'→3')	NCBI Gene Number	Anneal T_m
MMP13	F: ACCCTGGAGCACTCATGTTTCCTA R: TGGCATCAAGGGATAAGGAAGGGT	NM_002427.3	60°C
ADAMTS5	F: CTGTGACGGCATCATTGGCTCAAA R: TTCAGGAATCCTCACCACGTCAGT	NM_007038.3	60°C
ACAN	F: TCTTGGAGAAGGGAGTCCAACTCT R: ACAGCTGCAGTGATGACCCTCAGA	NM_001135.3	60°C
FGFR1	F: AACCTGACCACAGAATTGGAGGCT R: ATGCTGCCGTACTCATTCTCCACA	NM_023110.2	60°C
FGFR2	F: TGATGGACTTCCTTATGTCCGCGT R: AGCGTCCTCTTCTGTGACATTGGT	NM_000141.4	60°C
FGFR3	F: ACCAATGTGTCTTTCGAGGATGCG R: AGAGCACGCAGCTTGTCACATAGA	NM_000142.4	60°C
FGFR4	F: ATGGAACTGGTGTGCTCAAGAAGC R: TTCACATGTCCTCCGACCAACACA	NM_002011.3	60°C
18S rRNA	F: CGGCTACCACATCCAAGGAA R: GCTGGAATTACCGCGGCT	NR_003286.2	60°C
GAPDH	F: TCGACAGTCAGCCGCATCTTCTTT R: GCCCAATACGACCAAATCCGTTGA	NM_002046.3	60°C

ACAN, aggrecan core protein; ADAMTS5, a disintegrin and metalloproteinase with a thrombospondin type 1 motif 5; FGFR, fibroblast growth factor receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP13, matrix metalloproteinase-13.

ISSN: 1478-6362