Figure 6

Ipsilateral PLN and ILN collapse is associated with B cell translocation into LYVE+ sinuses. Ipsilateral pairs of PLN and ILN (n = 4) from the mice described in Figure 1 were processed for IHC with fluorescently labeled antibodies against IgM and LYVE-1 to image the B cells (red) and lymphatic endothelium (green) respectively. Multicolor fluorescent micrographs (5x) of representative ILN (A, B) and PLN (C, D) are presented to illustrate the distinct staining in expanding nodes (A, C), versus the apparent co-localization (yellow) in the collapsed nodes (B, D), due to the B cells that have translocated into the paracortical lymphatic sinuses. The images were analyzed in Image-Pro Plus and the percentage of yellow pixels representing the overlapping signal is indicated.