Figure 2From: Effects of short-term glucocorticoid treatment on changes in cartilage matrix degradation and chondrocyte gene expression induced by mechanical injury and inflammatory cytokinesEffects of Dex on GAG loss and chondrocyte biosynthesis in bovine cartilage treated with combinations of mechanical injury, TNFα and IL-6/sIL-6R. A) Percentage of GAG loss in bovine cartilage in response to six-day treatments. The mean ± SEM total GAG content was 466.3 ± 21.5 μg GAG/disk in the untreated control group. 10 nM DEX significantly reduced GAG loss from conditions involving TNFα plus IL-6/sIL-6R, mechanical injury or both. B) Chondrocyte biosynthetic rates measured by 35S-sulfate incorporation during days 4 to 6. TNFα, either with or without IL-6/sIL-6R and mechanical injury, significantly lowered biosynthesis of proteoglycan, while the addition of DEX to these conditions blocked the biosynthesis reductions. Values in A and B are presented as mean ± SEM. N = 10 cartilage disks in no-treatment control, DEX, TNFα, and DEX + TNFα conditions. N = 5 cartilage disks in the remaining conditions.* = P < 0.05 (only comparisons from selected hypothesis are shown). DEX, dexamethasone; GAG, glycosaminoglycans; IL-6, interleukin-6; SEM, standard error of the mean; sIL-6R, soluble interleukin-6 receptor; TNFα, tumor necrosis factor alpha.Back to article page