Figure 4From: Effects of short-term glucocorticoid treatment on changes in cartilage matrix degradation and chondrocyte gene expression induced by mechanical injury and inflammatory cytokinesDEX Pre and post treatment. A, Cumulative GAG loss and B, sulfate incorporation (measured in the last two days) from bovine cartilage samples pre-treated with 10 nM DEX for two days prior to a four-day TNFα treatment. On Day 4, Cartilage samples pre-incubated with DEX released significantly less GAG, and showed significantly higher proteoglycan synthesis in the TNFα treatment compared to samples without DEX pre-treatment. C, Cumulative GAG loss and D, sulfate incorporation (measured in the last two days) in bovine cartilage samples treated with TNFα, in the presence or absence of 10 nM DEX, with a two-day pre-exposure to TNFα. DEX treatment introduced after the TNFα pre-treatment showed significantly reduced GAG loss and increased sulfate incorporation on Day 4. In each condition, n = 5 cartilage disks. * = P < 0.05 (only comparing the GAG loss difference between conditions on Day 4). DEX: dexamethasone; GAG: glycosaminoglycans; TNFα: tumour necrosis factor alpha.Back to article page