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Table 2 Summary of differentially expressed genes in the lymph node cells of different groups of Lewis rats tested ex vivo and in vitroa

From: The gene expression profile of preclinical autoimmune arthritis and its modulation by a tolerogenic disease-protective antigenic challenge

  DEGs, n (%)
Characteristics Upregulation Downregulation Total
Ex vivo    
   Inc/Nv 322 (100%) 0 (0%) 322
   Pk/Nv 31 (53.4%) 27 (46.6%) 58
   Rec/Nv 28 (80%) 7 (20%) 35
In vitro    
   Preclinical arthritis rats 41 (67.2%) 20 (32.8%) 61
   Preclinical Bhsp65-tolerized rats 579 (98%) 12 (2%) 591
  1. aDEG: differentially expressed gene. "Ex vivo" group shows DEGs in lymph node cells (LNCs) at incubation (Inc), peak (Pk) or recovery (Rec) phase compared to naïve (Nv) state; "in vitro" group shows DEGs induced by mycobacterial heat shock protein 65 (Bhsp65) compared to baseline control (LNCs cultured in medium alone). In Bhsp65-tolerized group, only 76 (12.86%) of the 579 DEGs were related to immune activity (innate immunity, cell-mediated immunity and humoral immunity). The major groups comprised genes relating to cellular proliferation 127 (21.5%) and metabolism 137 (23.2%).