Multiparameter flow cytometric analysis of PMA/Ca-Io induced cytokine expression in CD4+ T-cells and CD8+ T-cells. Whole blood from SLE patients and healthy controls (HC) was stimulated with PMA/Ca-Io and analyzed for intracellular cytokine expression of IL-21 and IL-17A among CD4+ T-cells and CD8+ T-cells (A-F). Frequencies of these T-cells are shown. Horizontal lines represent the mean. P-values were calculated using the nonparametric Mann-Whitney U-test.