Dose-dependent effect of RB200 in collagen-induced arthritis. Following the onset of arthritis induced by bovine collagen, mice were treated intraperitoneally on the day of disease onset (day 1) and then on days 4 and 7 of disease with RB200 at a dose of 0.1 mg/kg (n = 6), 1 mg/kg (n = 8) or 10 mg/kg (n = 8). Control mice received an equivalent volume of PBS intraperitoneally (n = 6). (a) The increase in clinical score was assessed over a 10-day period, and the data are presented as means ± SEM compared to day 1 of arthritis. (b) Paw swelling was measured with callipers, and data are means ± SEM of both hind paws expressed expressing the difference from baseline (in millimetres). The data were derived from a representative experiment and analysed using two-way analysis of variance vs PBS-treated mice. ns = not significant; ***P < 0.001.