Detection of anti-nuclear reactivity in pp65
, SA-C3d or PBS immunized sera. HEp-2 substrate slides were used for detection of anti-nuclear antibodies. Sera from eight weeks post-immunization were 100X diluted for ANA stains. Patterns of speckle (a, i), chromatin (b, j), nucleosome (c, k), MSA I (d, l), MSA II (e, m) and centriole (f, n) were revealed with sera from pp65336-439-immunized mice. Nucleolar response (g, o) was detected in sera from pp651-167 or pp65336-439 immunized mice. Nuclear reactivity was not found in SA-C3d or PBS immunized mice (h, p). White arrowheads indicate the pattern of nuclear responses. MSA I/II: mitotic spindle type I/II.