Detection of anti-dsDNA antibody in pp65
, SA-C3d or PBS immunized sera. (a) ELISA assays for anti-dsDNA activity at 1:100 serum dilution. The dsDNA positivity is defined by mean + 3 s.e.m. of SA-C3d-immunized sera. O.D.450 > 0.49 was considered to be positive. (b) Isotyping of anti-dsDNA antibody for pp65336-439-immunized mice (n = 17) at eight weeks post-immunization. Tests were performed on ELISA at 1:100 dilution. dsDNA(+): dsDNA seropositive, dsDNA(-): dsDNA seronegative. Graphs depict mean ± s.e.m. values. Unpaired Student t test was performed. Results with a P-value of < 0.05 were considered to be significant. (c) Representations of Crithidia luciliae stain by sera from (c1) pp65336-439, (c2) pp651-167, (c3) SA-C3d or (c4) PBS immunized animals at eight weeks post-immunization at 1:40 dilution. White arrowheads indicate dsDNA positive stains. (d) The summarized results of ELISA assays and Crithidia luciliae stains. These results are representative of triplicated experiments.