Detection of protein-to-protein interaction between pp65
and HeLa lysate. Whole HeLa lysate was separated by SDS-PAGE and transferred to nitrocellulose paper. The pp65336-439 or pp651-167 His-tag fusion peptide was incubated with blot at concentrations of 20, 10, 5, 2.5, 1.25, 0.625 mg/ml (No.1 to 6) or at concentrations of 20, 10, 5, 2.5 mg/ml (No.7 to 10), respectively. Asterisk indicates protein-free negative control. Molecular mass markers (kD) are shown on the left. MW: molecular weight (kD).