Skip to main content

Table 1 Primers sets for the truncation of HCMVpp65 antigen constructions

From: Fragment of tegument protein pp65 of human cytomegalovirus induces autoantibodies in BALB/c mice

Clones Sequences Vectors
  Forward (5- > 3) Reverse (5- > 3)  
pp651-167 ATG GAT CCA TGG AGT CGC GCG GTC GCC G CCG GAA TTC CAG TCC CGA GAC CGT GAG GAC CGT pET30
pp65167-336 CGC GGA TCC TGG ACG CGT CAG CAG ACC CA CGC GGA TCC CTC GCG TAT GGC TTG TAC CT pET30
pp65336-561 CGC GGA TCC ACC GTG GAA CTG CGT CAG TA TAG GAT CCA CCT CGG TGC TTT TTG GGC G pET30
pp65336-448 CGC GGA TCC ACC GTG GAA CTG CGT CAG TA CGC CTC GAG CGA CGT GCA CGC CGT CGC pET30
pp65336-439 CGC GGA TCC ACC GTG GAA CTG CGT CAG TA CGC CTC GAG TGA TTT GCG TTT GCG GCC pET30
pp65336-422 CGC GGA TCC ACC GTG GAA CTG CGT CAG TA CGC CTC GAG GCC GGTGAC GCG GGG CGT pET30
murine C3d CGC GAT ATC ACC CCC GCA GGC TGT GGG GAA C 3' CGC GGA TCC GGA TCC GCT ACG GCT GGG GAG pET32
pp65336-439 CGC GGA TCC ACC GTG GAA CTG CGT CAG TA CGC CTC GAG TGA TTT GCG TTT GCG GCC pAS-1
  1. The underlined sequences mean the usage of restrictive enzyme