Table 1 Primers sets for the truncation of HCMVpp65 antigen constructions
From: Fragment of tegument protein pp65 of human cytomegalovirus induces autoantibodies in BALB/c mice
Clones | Sequences | Vectors | |
|---|---|---|---|
Forward (5- > 3) | Reverse (5- > 3) | ||
pp651-167 | ATG GAT CCA TGG AGT CGC GCG GTC GCC G | CCG GAA TTC CAG TCC CGA GAC CGT GAG GAC CGT | pET30 |
pp65167-336 | CGC GGA TCC TGG ACG CGT CAG CAG ACC CA | CGC GGA TCC CTC GCG TAT GGC TTG TAC CT | pET30 |
pp65336-561 | CGC GGA TCC ACC GTG GAA CTG CGT CAG TA | TAG GAT CCA CCT CGG TGC TTT TTG GGC G | pET30 |
pp65336-448 | CGC GGA TCC ACC GTG GAA CTG CGT CAG TA | CGC CTC GAG CGA CGT GCA CGC CGT CGC | pET30 |
pp65336-439 | CGC GGA TCC ACC GTG GAA CTG CGT CAG TA | CGC CTC GAG TGA TTT GCG TTT GCG GCC | pET30 |
pp65336-422 | CGC GGA TCC ACC GTG GAA CTG CGT CAG TA | CGC CTC GAG GCC GGTGAC GCG GGG CGT | pET30 |
murine C3d | CGC GAT ATC ACC CCC GCA GGC TGT GGG GAA C 3' | CGC GGA TCC GGA TCC GCT ACG GCT GGG GAG | pET32 |
pp65336-439 | CGC GGA TCC ACC GTG GAA CTG CGT CAG TA | CGC CTC GAG TGA TTT GCG TTT GCG GCC | pAS-1 |