Table 1 Primers used for real-time reverse transcription-polymerase chain reaction
From: An organ culture system to model early degenerative changes of the intervertebral disc
Target genes | Sequence (5' to 3') | Size, base pairs |
|---|---|---|
| Â | F: TGGCCAAGAAGACATCCCTGAAGT | Â |
Collagen type 1 | R: ACATCAGGTTTCCACGTCTCACCA | 81 |
| Â | F: GAGTGGAAGAGCGGAGACTACTG | Â |
Collagen type 2 | R: CTCCATGTTGCAGAAGACTTTCA | 81 |
| Â | F: TTCATGCATCCGCTAGTCCCTTCT | Â |
Collagen type 6 | R: CCTTTATTGGGCCATTGGGCATGT | 136 |
| Â | F: TCGTGGATGTGGTGCTGAAGATGA | Â |
Collagen type 9 | R: ATTGGGTCCCTGTTTGCCTGGATA | 146 |
| Â | F: TGTCCAACAACCTGTGGAGGAAGA | Â |
Fibromodulin | R: CGTTGTGTGTGTGTGTGTGTGTGT | 113 |
| Â | F: AAGGACTGTCTATCTGCACGCCAA | Â |
Aggrecan | R: TCACCACCCACTCCGAAGAAGTTT | 126 |
| Â | F: ATTGGCACAAAGGTGGATGCTGTC | Â |
MMP-3 | R: ACGACGCCTTCCATGGATCTTCTT | 160 |
| Â | F: TTCTCGAATCACGGAGGAAGCCAA | Â |
MMP-9 | R: AAGGCTGAGTTCAACTTTGCAGGC | 89 |
| Â | F: ACTTGTGTGACAGGAGCTAAGGCA | Â |
MMP-13 | R: AGGAGCATGAAAGGGTGGTCTCAA | 119 |
| Â | F: CTGGCCAATGGCATGGATCTCAAA | Â |
TNF-α | R: ATGAAATGGCAAATCGGCTGACGG | 148 |
| Â | F: ACCTGCTAGTGTGTGATGTTCCCA | Â |
IL-1β | R: AGGTGGAGAGCTTTCAGCTCACAT | 109 |
| Â | F: ACTTCCAGGCCCATGGTACAATCT | Â |
NGF | R: TTGATGTCCGTGGCTGTGGTCTTA | 148 |
| Â | F: AGTCCCTGCCCTTTGTACACA | Â |
18S | R: GATCCGAGGGCCTCACTAAAC | 68 |