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Centromere
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Centromeric immunofluorescence pattern on HEp-2 cells (308 of 310 were positive) or a CENP-B band in line assay (309 of 310 positive)
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|
Topoisomerase I
|
At least two of three findings: Scl-70-positive signal in line assay (258 of 260 positive), a band comigrating with a prototype band in IP (all positive), a line of identity in ID with the Scl-70 prototype serum (244 of 260 positive)
|
Typical HEp-2 cell immunofluorescence pattern: fine granular karyoplasmic, weakly nucleolar, metaphase chromosome-positive
|
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RNA polymerases
|
Characteristic IP pattern comigrating with the pattern of a prototype serum mainly consisting of four bands: Ia, Ib, IIIa and IIIb [58]
|
Confirmation by ELISA in 32 of 32 cases with the immunodominant epitope of RNA polymerase III subunit RPC155 according to Kuwana et al. [71], provided by Matritec, Freiburg, Germany. ANA immunofluorescence on HEp-2 cells was predominantly fine granular only sometimes (five cases) in addition nucleolar [72].
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Fibrillarin
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An IP band (approximately 34 kDa) comigrating with a prototype serum band, plus a nucleolar immunofluorescence pattern on HEp-2 cells
|
Confirmation by investigational ELISA kindly provided by Euroimmun, Lübeck, Germany, positive in 11 and borderline in 1 of the 12 cases
|
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To
|
An IP band of approximately 40 kDa plus a nucleolar immunofluorescence pattern on HEp-2 cells; confirmation by immunoblot analysis with recombinant To antigen kindly supplied by Dr M Blüthner, Labor Seelig, Karlsruhe, Germany
| |
|
PM-Scl
|
A line of identity in ID with a PM-Scl prototype serum (41 of 42 cases) and/or positive result of ELISA with the synthetic peptide PM-1α [73] (Dr Fooke Laboratorien GmbH, Neuss, Germany) (12 of 13 cases)
|
Positive reaction in 37 of 41 cases for PM-Scl by line assay. ANA immunofluorescence on HEp-2 cells usually was nucleolar plus fine granular karyoplasmic.
|
|
Ku
|
Two prominent IP bands at about 70 and 80 kDa comigrating with prototype bands
|
In 3 of 10 cases, a line identical to a Ku prototype band in ID. ANA immunofluorescence was finely granular, usually at a high titre.
|
|
U1-RNP
|
A positive signal for RNP/Sm in line assay, with or without a positive signal for Sm, plus a typical IP pattern consisting of at least antigen A (about 33 kDa), antigen B/B' (about 28/29 kDa) and antigen C (about 22 kDa)
|
In 37 of 41 cases, a line of identity with a U1-RNP prototype in ID. ANA pattern on HEp-2 cells usually was coarsely speckled.
|
|
Sm
|
A positive signal for RNP/Sm as well as for Sm in line assay
|
In two of four cases, a band identical to a Sm prototype in ID with ribonuclease-digested calf thymus extract. IP and immunofluorescence patterns were similar to those found for anti-U1-RNP.
|
|
Jo-1
|
A positive signal for Jo-1 in line assay plus a band identical to a Jo-1 prototype band in ID
|
Immunofluorescence on HEp-2 cells was inconsistent.
|
|
Pl-7
|
IP band of about 80 kDa comigrating with prototype band plus a band identical to a Pl-7 prototype band in ID
|
Cytoplasmic immunofluorescence on HEp-2 cells
|
|
OJ
|
Typical triplet band in IP comigrating with prototype bands
|
Cytoplasmic immunofluorescence on HEp-2 cells
|
|
U11-RNP [74, 75]
|
An RNP-like IP pattern and coarsely speckled ANA immunofluorescence, without any U1-RNP signals in line assay and ID; U11-RNP specificity detected by C Will and R Lührmann, Marburg, Germany
| |
|
p25/p23 [76, 77]
|
Doublet IP bands of about 25 and 23 kDa, with the 25 kDa band comigrating with the precipitate of rabbit anti-p25 kindly provided by E Chan, Gainesville, FL, USA
|
HEp-2 cell immunofluorescence pattern was always centromeric because anti-p25/p23 was exclusively found together with anticentromere.
|
|
SL
|
A band identical to the SL prototype band in ID plus an IP band at about 31 kDa comigrating with the precipitate of the SL reference serum
|
HEp-2 cell immunofluorescence pattern was fine granular, but in this study often was masked because of other coexisting antibodies
|
|
NOR-90
|
Doublet IP bands at about 90 kDa comigrating with the precipitate of a NOR-90 reference serum [78]
|
The nucleolar immunofluorescence pattern expected on HEp-2 cells was hard to detect in the sera examined in this study, because NOR-90 antibodies in all cases coincided with other autoantibodies visible on HEp-2 cells.
|
|
Mitochondrial M2
|
AMA M2-positive signal by line assay (40 of 41 positive) and/or AMA typical cytoplasmic immunofluorescence on HEp-2 cells and/or rat kidney sections (27 of 41 positive)
|
In IP, a band of around 70 kDa was present in 36 of 41 cases.
|
|
Sp100
|
Multiple nuclear dot pattern on HEp-2 cells [79] plus Sp100 signal in the line assay HUMAN IMTEC-Liver Line Immunoassay (HUMAN Diagnostics GmbH, Wiesbaden, Germany)
| |
|
Ro52
|
Ro52-positive signal by line assay
| |
|
Ro60
|
Ro60-positive signal by line assay
| |
|
La
|
La-positive signal by line assay
| |
