Figure 5

In the presence of serum, IL-1 and TNF-α decreased, while TGF-β1 increased, cell proliferation. (A) Representative confocal images of the micro-wound from inner zone cells that were treated with 10% serum, 10% serum + 10 ng/mL IL-1, 10% serum + 10 ng/mL TNF-α, or 10% serum + 10 ng/mL TGF-β1 for 48 hours after the scratch. In the confocal images, cells that have proliferated are yellow and all cells are labeled red. The scale bar is equal to 100 μm. (B) For inner zone cells, total cells in the wound, proliferated cells in the wound, migrated cells in the wound, and proliferated cells at the edge at 24 and 48 hours are graphed as a percentage + standard error of the starting cell density at the edge of the scratch. a: P < 0.05 compared to 10% serum at 48 hour; b: P < 0.05 compared to serum + TGF-β1 at 48 hours; c: P < 0.05 compared to serum + TNF-α at 24 hours. (C) Representative confocal images of the micro-wound from outer zone cells that were treated with 10% serum, 10% serum + 10 ng/mL IL-1, 10% serum + 10 ng/mL TNF-α, or 10% serum + 10 ng/mL TGF-β1 for 48 hours after the scratch. (D) For outer zone cells, total cells in the wound, proliferated cells in the wound, migrated cells in the wound, and proliferated cells at the edge at 24 and 48 hours are graphed as a percentage + standard error of the starting cell density at the edge of the scratch.