Treatments | Concentrations | Vendor |
---|---|---|
Serum | 0, 1%, 5%, 10% | Hyclone (Logan, UT, USA) |
Recombinant porcine IL-1α | 0, 0.1 ng/mL, 1 ng/mL, 10 ng/mL | R & D Systems (Minneapolis, MN, USA) |
Recombinant porcine TNF-α | 0, 0.1 ng/mL, 1 ng/mL, 10 ng/mL | R & D Systems (Minneapolis, MN, USA) |
Porcine TGF-β1 | 0, 0.1 ng/mL, 1 ng/mL, 10 ng/mL | R & D Systems(Minneapolis, MN, USA) |
10% Serum and factors | 10% Serum | Hyclone (Logan, UT, USA) |
 | 10% Serum + 10 ng/mL IL-1 | see above |
 | 10% Serum + 10 ng/mL TNF-α | see above |
 | 10% Serum + 10 ng/mL TGF-β1 | see above |