Representative 2D map of primary Sjögren's syndrome (pSS) (A) and healthy subject (B). A total of 150 µg of whole saliva proteins was separated by 2D using 18 cm pH 3 to 10 L strip (IPG) and 12.5% SDS-PAGE. Proteins were detected by silver staining. The map was analysed by Image master 2D platinum software. Spot numbers indicate all the proteins identified by MS/MS and refer to the number reported in Tables 2-4.