Representative 2D map of secondary Sjögren's syndrome and other sicca syndromes. (A) non-SS sicca syndrome, (B) secondary Sjögren's syndrome concomitant to rheumatoid arthritis RA-sSS and (C) secondary Sjögren's syndrome concomitant to systemic sclerosis SSc-sSS whole saliva proteins. A total of 150 µg of proteins was separated by 2D using 18 cm pH 3 to 10 L strip (IPG) and 12.5% SDS-PAGE. Proteins were detected by silver staining. The map was analysed by Image master 2D platinum software. Spot numbers indicate all the proteins identified by MS/MS and refer to the number reported in Tables 2-4.