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Table 2 Adenosine receptor binding parameters in lymphocyte membranes from healthy controls, ERA and RA patients

From: A2A and A3 adenosine receptor expression in rheumatoid arthritis: upregulation, inverse correlation with disease activity score and suppression of inflammatory cytokine and metalloproteinase release

 

(3H)-DPCPX

A1ARs

(3H)-ZM 241385

A2AARs

(3H)-MRE2029F20

A2BARs

(3H)-MRE3008F20

A3ARs

Healthy

controls

n = 90

KD = 1.76 ± 0.08

nM

Bmax = 34 ± 2 fmol/mg protein

KD = 1.41 ± 0.10 nM

Bmax = 58 ± 4

fmol/mg protein

KD = 2.29 ± 0.14 nM

Bmax = 55 ± 3

fmol/mg protein

KD = 1.92 ± 0.09 nM

Bmax = 137 ± 9

fmol/mg protein

ERA patients

n = 32

KD = 1.74 ± 0.06

nM

Bmax = 33 ± 2 fmol/mg protein

KD = 2.09 ± 0.14* nM

Bmax = 185 ± 12* fmol/mg protein

KD = 2.29 ± 0.10 nM

Bmax = 55 ± 2 fmol/mg protein

KD = 3.03 ± 0.14* nM

Bmax = 290 ± 15*

fmol/mg protein

RA

patients

n = 63

KD = 1.69 ± 0.07

nM

Bmax = 35 ± 2 fmol/mg protein

KD = 1.93 ± 0.12* nM

Bmax = 195 ± 13* fmol/mg protein

KD = 2.36 ± 0.07 nM

Bmax = 54 ± 2 fmol/mg protein

KD = 3.47 ± 0.21* nM

Bmax = 328 ± 22* fmol/mg protein

  1. ERA, early rheumatoid arthritis; KD, affinity constant, nM; Bmax, maximum density of specific binding sites, fmol/mg protein; RA, rheumatoid arthritis. Data are expressed as mean ± SEM. Differences were considered significant at a value of P < 0.01 vs healthy controls (*).