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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis

Figure 3

Recombinant human SIRT1 transfection into nucleus pulposus cells. Transfection of recombinant human silent mating type information regulator 2 homolog 1 (rhSIRT1) labeled with HiLyte Fluor™ 555 by Pro-DeliverIN™ into human nucleus pulposus (NP) cells. (a) Immunohistochemical staining for SIRT1, β-actin and the nucleus (4',6-diamidino-2-phenylindole). Red fluorescence shows labeled rhSIRT1 and green fluorescence shows β-actin. Arrows show labeled rhSIRT1. Of the NP cells, 37.9% were transfected with labeled rhSIRT1. Bars = 20 μm. (b) Western blot analysis for acetylated p53 and total p53. Western blot analysis confirmed that transfected SIRT1 deacetylated p53.

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