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Table 1 Gene-specific primer sequences used in RT-PCR and quantitative real-time RT-PCR

From: Expression of silent mating type information regulator 2 homolog 1 and its role in human intervertebral disc cell homeostasis

Gene Primer sequence Accession number
SIRT1 Forward: 5'-GCC TCA CAT GCA AGC TCT AGT GAC-3' GenBank:NM_012238
  Reverse: 5'-TTC GAG GAT CTG TGC CAA TCA TAA-3'  
Aggrecan Forward: 5'-CTA CCA GTG GAT CGG CCT GAA-3' GenBank:NM_001135
  Reverse: 5'-CGT GCC AGA TCA TCA CCA CA-3'  
Sox9 Forward: 5'-GGA GAT GAA ATC TGT TCT GGG AAT G-3' GenBank:NM_000346
  Reverse: 5'-TGA AGG TTA ACT GCT GGT GTT CTG A-3'  
Collagen type 2 Forward: 5'-AAG GTG CTT CTG GTC CTG CTG-3' GenBank:NM_001844
  Reverse: 5'-GGG ATT CCA TTA GCA CCA TCT TTG-3'  
α 2 -Macroglobulin Forward: 5'-TGG CGA TCG TTG ATG TGA AGA-3' GenBank:NM_000014.4
  Reverse: 5'-CTG TCC GGC TCA CAT GGT TAG A-3'  
Cytokeratin-18 Forward: 5'-AGG AGT ATG AGG CCC TGC TGA A-3' GenBank:NM_000224.2
  Reverse: 5'-TTG CAT GGA GTT GCT GCT GTC-3'  
CD56 Forward: 5'-TTC AAA TGC ACA GGC CCT CA-3' GenBank:NM_000615.5
  Reverse: 5'-GTC TCA GAC ACC CAG GCT CTC A-3'  
GAPDH Forward: 5'-GCA CCG TCA AGG CTG AGA AC-3' GenBank:NM_002046
  Reverse: 5'-TGG TGA AGA CGC CAG TGG A-3'  
  1. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SIRT1, silent mating type information regulator 2 homolog 1.