Table 1 Gene-specific primer sequences used in RT-PCR and quantitative real-time RT-PCR

SIRT1

Forward: 5'-GCC TCA CAT GCA AGC TCT AGT GAC-3'

GenBank:NM_012238

Reverse: 5'-TTC GAG GAT CTG TGC CAA TCA TAA-3'

Aggrecan

Forward: 5'-CTA CCA GTG GAT CGG CCT GAA-3'

GenBank:NM_001135

Reverse: 5'-CGT GCC AGA TCA TCA CCA CA-3'

Sox9

Forward: 5'-GGA GAT GAA ATC TGT TCT GGG AAT G-3'

GenBank:NM_000346

Reverse: 5'-TGA AGG TTA ACT GCT GGT GTT CTG A-3'

Collagen type 2

Forward: 5'-AAG GTG CTT CTG GTC CTG CTG-3'

GenBank:NM_001844

Reverse: 5'-GGG ATT CCA TTA GCA CCA TCT TTG-3'

α ₂ -Macroglobulin

Forward: 5'-TGG CGA TCG TTG ATG TGA AGA-3'

GenBank:NM_000014.4

Reverse: 5'-CTG TCC GGC TCA CAT GGT TAG A-3'

Cytokeratin-18

Forward: 5'-AGG AGT ATG AGG CCC TGC TGA A-3'

GenBank:NM_000224.2

Reverse: 5'-TTG CAT GGA GTT GCT GCT GTC-3'

CD56

Forward: 5'-TTC AAA TGC ACA GGC CCT CA-3'

GenBank:NM_000615.5

Reverse: 5'-GTC TCA GAC ACC CAG GCT CTC A-3'

GAPDH

Forward: 5'-GCA CCG TCA AGG CTG AGA AC-3'

GenBank:NM_002046

Reverse: 5'-TGG TGA AGA CGC CAG TGG A-3'